Antigen

Antigen Class

TFs and others

Antigen

MYOD1

Cell type

Cell type Class

Muscle

Cell type

RD

Primary Tissue

Skelatal Muscle

Tissue Diagnosis

Rhabdomyosarcoma

Sample

source_name

RD

cell line

RD

cell type

Fusion-Negative Rhabdomyosarcoma

genotype

WT

chip-antibody

MYOD (CST, #13812)

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM7720563

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixes with 1% formaldehyde for 10 min. After quenching, cells were digested to achieve chromatin fragmented to a range of 150–900 bp using Micrococcal Nuclease. Chromatin samples were immunoprecipitated overnight at 4 °C. DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep kit, following manifacturer's instruction.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

30457754

Reads aligned (%)

87.8

Duplicates removed (%)

9.7

Number of peaks

1050 (qval < 1E-05)

hg19

Number of total reads

30457754

Reads aligned (%)

87.2

Duplicates removed (%)

10.1

Number of peaks

778 (qval < 1E-05)